Detection of plasmid DNA

Detection of contaminating plasmid DNA by PCR

A polymerase chain reaction (PCR) test can be used to qualitatively detect certain DNA gene sequences. In this way, the residual plasmid DNA, which is transported into the body cells via the lipid nanoparticles at the same time as the vaccine modRNA, can be visualised. This test should only be considered if spike proteins have already been detected in the tissue – especially in tumour tissue. In principle, PCR can be performed on all of the above-mentioned test materials.

Why is the detection of plasmid DNA in tumour tissue important?

As already mentioned, the gene products contain significant amounts of plasmid DNA after the manufacturing process. To remove this, a DNase-I reaction was applied to the unfinished product to break up the plasmid DNA and then remove it by filtration. However, large quantities of small, now linear DNA fragments remained in the final product, which are then transported into the body cells during injection. An undetermined proportion of these are released into the cytoplasm and enter the cell nucleus. It is scientifically known that small, linear DNA fragments can integrate into the genetic material of the cell.

The integration of foreign DNA occurs preferentially at sites where genes are actively transcribed. In particular, genes that are responsible for cell regulation, DNA repair and cell metabolism are of risk of being altered in expression levels or will be destroyed by integration. Such DNA integration can demonstrably lead to the formation of a tumour cell. If this tumour cell survives, a malignant cancer can be induced. In this case, the COVID injection would be responsible for the development of a tumour.

If we find one or more DNA fragments in a tumour biopsy, it is possible to detect the integration sites using highly developed special technologies. We would then do this in collaboration with our genome experts.